The fate of human malignant melanoma cells transplanted into. In zebrafish, the mature notochord consists of a single stack of fully. Vimentin antibody rabbit polyclonal from proteintech validated in western blot wb, immunohistochemistry ihc, immunofluorescence if, flow cytometry fc, enzymelinked immunosorbent assay elisa applications. Transglutaminasemediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may. Global supplier of antibodies, proteins and elisa kits 25 years expertise in serving research and diagnostic customers. Vimentin antibody mouse anti human vim fitc prospec. Vimentin v9is also recommended for detection of vimentin in additional species, including porcine. Vimentin antibody used for immunofluorescence on cryosections of mouse, canine and human quadriceps muscle at a dilution of 1. It is aimed to help labome visitors find the most suited vimentin antibody. Vimentin antibody c20 scbt santa cruz biotechnology. The vimentin antibody from proteintech is a rabbit polyclonal antibody to a fusion protein of human vimentin. Rabbit monoclonal epr3776 to vimentin cytoskeleton marker alexa. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. C crosssection through a 3d larvae eye labeled with antipax6 antibody brown and a conespecific antibody, fret43 black.
Sequential immunofluorescence and immunohistochemistry. D oblique section through the surface of the optic nerve of an adult zebrafish labeled with anti pax2 antibody in yellow and anticytokeratin antibody in green and visualized using confocal microscopy. Oct4 antibody is directed against synthetic peptide aa282297 of. Further information about each individual vimentin antibody is available by following the associated links. Compare anti vimentin antibody products from leading suppliers on biocompare.
We appreciate as much detail as possible and references as appropriate. Some distinctive features of zebrafish myogenesis based on. Chicken polyclonal antivimentin antibody mesenchymal cells marker cited in 38 publications. Vimentin is a developmentally regulated intermediate filament protein ifp found in cells of mesenchymal origin. Vimentin as a marker for epithelialtomesenchymal transition. There are 3 vimentin antibody for wb, 4 vimentin antibody for ihcp. The hybridomas are stored at the monoclonal antibody facility of the uo under the supervision of dr. Vimentin antibody staining protocol for immunohistochemistry. Vimentin antibody customerized service is available.
In the embryonic nervous system, however, vimentin is expressed by migrating neural crest cells forming the pns, and by neural stem cells of the cns. Compare antivimentin antibody products from leading suppliers on biocompare. The role of vimentin intermediate filaments in the. I am planning on using mouse monoclonal vimentin antibody 2d1, nbp192687 on mouse fibroblasts. The intermediate filament protein vimentin is a new target for epigallocatechin gallate received for publication, december 17, 2004, and in revised form, february 8, 2005 published, jbc papers in press. Vimentin antibody rabbit polyclonal from proteintech validated in western blot wb, immunohistochemistry ihc, immunofluorescence if, flow cytometry fc, enzymelinked. It was initially shown in 2001 that heart transplant patients produce igm antivimentin antibodies within 2 years of transplantation and that higher titres in the first two years. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. The vimentin antibody has been validated for the following applications. Emt related circular rna expression profiles identify circscyl2 as a novel molecule. Vimentin is an intermediate filament that is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells. Vimentin is useful as part of an antibody panel for differential diagnostic purposes, such as differentiating soft tissue.
Access journals video booksebooks trade magazines enewsletters. Vimentin antibody v9 scbt santa cruz biotechnology. Applications key ab array antibody array ae affinity electrophoresis affin chroma. Vimentin is a well established marker of early differentiating neuronal lineage cells. Vim antibody mouse anti human vimentin antibody prospec. This antibody recognizes human, mouse, and rat antigen. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and other diseases.
In the vertebrate heart, antivimentin antibodies react with the abundant. The zebrafish heart regenerates after cryoinjuryinduced myocardial. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. The vimentin antibody sampler kit provides an economical means to detect total levels of vimentin, vimentin phosphorylated at ser56, and vimentin phosphorylated at ser82.
Characterization of proliferating neural progenitors after spinal. The kit contains enough primary and secondary antibody to perform two western blot experiments. Ihc staining of zebrafish for cytokeratin is impacted by fixative or length of. Vimentin was detected in immersion fixed k562 human chronic myelogenous leukemia cell line but is not detected in vimentin. Vimentin antibody rv202 nbp197672 staining of a 1 month old zebrafish embryo immunohistochemistryfrozen. Some steps, like fusion before striation, seem to be different in the zebrafish when compared. Antibody ab8978 antivimentin antibody rv202 by abcam. As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in.
The absence of signal in the vimentin knockout hela cells confirms specificity of the antibody for vimentin. Vimentin is an intermediate filament protein which is characteristically upregulated in cells undergoing emt. Among all these vimentin antibodies, there are 2 anti vimentin rabbit monoclonal antibodies, 3 anti vimentin rabbit polyclonal antibodies. Role of antivimentin antibodies in allograft rejection. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Vimentin can be phosphorylated by akt1, a kinase activated downstream of ras and pi3k, and phosphorylation of vimentin protects it from caspaseinduced proteolysis and enhances cell migration and metastasis. Zebrafish antibodies primary antibodies sigmaaldrich. Synthetic peptide surrounding amino acid 448 of human vimentin. Antivimentin antibody epr3776 cytoskeleton marker alexa fluor 594 ab154207 specific references 6 description.
The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the specific staining patterns obtained with particular clones. A vimentin is necessary for emt induction downstream of slug and hrasv12. Antibody 5505 purified mouse antivimentin by bd biosciences. Here, we have established a heart infarct model in zebrafish using cryoinjury. Epithelialtomesenchymal transition emt is a critical event in the induction of cell motility and increased survival both under physiological situations like wound healing or development, as well as in malignant cells undergoing invasion and metastasis.
Vimentin antibody pa527231 thermo fisher scientific. Suitable for use as control antibody for vimentin sirna h. Vimentin is an intermediate filament that is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. It was found that cells without vimentin are extremely delicate when disturbed with a micropuncture. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane.
Jul 08, 20 vimentin is encoded by a singlecopy gene and is located on the short arm of chromosome 10, specifically at chromosome 10p. The vimentin v9 antibody from santa cruz biotechnology, inc. Among all these vimentin antibodies, there are 2 antivimentin rabbit monoclonal antibodies, 3 antivimentin rabbit polyclonal. Zebrafish can repair their injured brain and spinal cord after injury unlike adult. Vimentin elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either. Human vimentin is a 53,521 dalton protein 466 amino acids expressed in many cell types of mesodermal origin, and is one of the intermediate filament types that form the cytoskeleton. Vimentin specificity is shown by immunocytochemistry in knockout cell line. If proteins are found in all animal cells as well as bacteria. A 28h wholemount zebrafish embryo labeled with an antibody which recognizes the transcription factor, pax6 brown. Immunohistochemistry of wholemount and sectioned zebrafish tissue. The vimentin promoter is composed of many elements, including a tata box, eight putative gc boxes, activator protein 1 ap1 binding sites, the nf. Due to their striking genomic similarity to humans, regenerative abilities.
Libraries of zebrafishspecific antibodies are now available, but few. Further information about each individual vimentin antibody is. If, along with tubulinbased microtubules and actin. Proteins were transferred to a membrane and probed with a vimentin polyclonal. If, along with tubulinbased microtubules and actinbased microfilaments, comprises the cytoskeleton. Please use this form to recommend updates to the information in zfin. Vimentin v9 is a mouse monoclonal antibody raised against purified vimentin from eye lens of porcine origin. The intermediate filament network changes from being composed of vimentin to. Islands of hn2 cells form after 4 days in culture forming beautiful flower like structures. Hela cells were fixed with 4% paraformaldehyde for 30 min at rt, permeabilized with 0.
Lowpower immunofluorescence image of a 24h zebrafish embryo tail somites 1830 using anti. A model indicating an important role for vimentin as a positive regulator of gene expression and morphological changes in. Arg66199 antivimentin antibody sqab1721 iccif image. Evaluating the effects of various decalcification protocols on. It is believed to be involved with the intracellular transport of proteins. Is expressed in several structures, including axis. Transglutaminasemediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Vimentin is a strong arterial substrate for transglutaminases. All the vimentin anbodies are produced in house and all are in stock. Measures igg antibodies to mutated citrullinated vimentin mcv. Higher magnification images of human melanoma cell stained with vimentin antibody in the 3. The vimentin antibody from proteintech is a rabbit polyclonal antibody to a recombinant protein of human vimentin. A novel model of cryoinjuryinduced myocardial infarction in the.
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